eIF3 p110 Antibody (B-6): sc-74507

eIF3 p110 Antibody (B-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects eIF3 p110 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eIF3 p110 antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF3 p110 plays a crucial role in the initiation of translation, a fundamental process for protein synthesis in eukaryotic cells. eIF3 p110 functions as a key component of the eukaryotic initiation factor 3 (eIF3) complex, which is essential for the formation of the 48S ribosomal complex. This complex ensures accurate recognition of the start codon on mRNA, enabling correct translation initiation. eIF3 p110 helps stabilize the association of the eIF2-GTP-Met-tRNAiMet complex with the 40S ribosomal subunit, facilitating mRNA binding and promoting the dissociation of 80S ribosomes into their 40S and 60S subunits. Given this pivotal role in translation initiation, eIF3 p110 remains vital for cellular function and growth, making eIF3 p110 an important target for research in various biological and disease processes.

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eIF3 p110 Antibody (B-6) References:

1. Distinct functions of eukaryotic translation initiation factors eIF1A and eIF3 in the formation of the 40 S ribosomal preinitiation complex.  |  Chaudhuri, J., et al. 1999. J Biol Chem. 274: 17975-80. PMID: 10364246
2. Protein 4.1R binding to eIF3-p44 suggests an interaction between the cytoskeletal network and the translation apparatus.  |  Hou, CL., et al. 2000. Blood. 96: 747-53. PMID: 10887144
3. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo.  |  Asano, K., et al. 2000. Genes Dev. 14: 2534-46. PMID: 11018020
4. Mutually cooperative binding of eukaryotic translation initiation factor (eIF) 3 and eIF4A to human eIF4G-1.  |  Korneeva, NL., et al. 2000. J Biol Chem. 275: 41369-76. PMID: 11022043
5. Eukaryotic initiation factor 3 p110 mRNA is overexpressed in testicular seminomas.  |  Rothe, M., et al. 2000. Am J Pathol. 157: 1597-604. PMID: 11073819
6. Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding.  |  Valásek, L., et al. 2001. EMBO J. 20: 891-904. PMID: 11179233
7. Human DDX3 functions in translation and interacts with the translation initiation factor eIF3.  |  Lee, CS., et al. 2008. Nucleic Acids Res. 36: 4708-18. PMID: 18628297
8. Identification of a novel autoantigen eukaryotic initiation factor 3 associated with polymyositis.  |  Betteridge, Z., et al. 2020. Rheumatology (Oxford). 59: 1026-1030. PMID: 31728542
9. Conservation and diversity of eukaryotic translation initiation factor eIF3.  |  Asano, K., et al. 1997. J Biol Chem. 272: 1101-9. PMID: 8995409
10. Structure of cDNAs encoding human eukaryotic initiation factor 3 subunits. Possible roles in RNA binding and macromolecular assembly.  |  Asano, K., et al. 1997. J Biol Chem. 272: 27042-52. PMID: 9341143
11. Biochemical characterization of mammalian translation initiation factor 3 (eIF3). Molecular cloning reveals that p110 subunit is the mammalian homologue of Saccharomyces cerevisiae protein Prt1.  |  Chaudhuri, J., et al. 1997. J Biol Chem. 272: 30975-83. PMID: 9388245
12. Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor eIF3.  |  Block, KL., et al. 1998. J Biol Chem. 273: 31901-8. PMID: 9822659

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF3 p110 Antibody (B-6) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF3 p110 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF3 p110 Antibody (B-6) Specifications

Alternate Product Name: eIF3 p110

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 611-761

Epitope Target: eIF3 p110

Epitope Species: human

Product Reactivity/Detection: eIF3 p110

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for eIF3 p110 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin